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Generation of Knockout Plasmids and Transfections Two different plasmids (pL0001 and pL0034) were used to create a total of four knockout plasmids (Figure 1) to attempt disruption of the pbgst gene

10.1192/bjp.bp.111.107664 8 AzharM.ZengG.AhmedA.Dar FarooqA.ChoudharyM

SAR and QSAR in Environmental Research 29 , 785800, (2018)

Formula Dosage (mcg) = (Vial mg Total ml) Injection volume (ml) 1000 Example Calculation Vial: 5 mg GHK-Cu Water added: 2 ml Injection volume: 0.1 ml (5 mg 2 ml) = 2.5 mg/ml 2.5 mg/ml 0.1 ml = 0.25 mg 0.25 mg 1000 = 250 mcg Therefore, 0.1 ml equals 250 mcg

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